Fukuchi T
Department of Ophthalmology, Niigata University, School of Medicine, Japan.
Nippon Ganka Gakkai Zasshi. 1990 Nov;94(11):1024-30.
The presence and distribution of collagen type I through VI, laminin, fibronectin and alpha-elastin were demonstrated in the normal monkey optic nerve lamina cribrosa, using a immunohistochemical method, the biotin-streptavidin (B-SA) system. Interstitial collagens, collagen type I and III, were detected diffusely within laminar beams continuously with surrounding sclera. The adventitia around the central artery and vein, and pial septa were also stained with collagen type I and III. Collagen type IV and laminin, which were basement membrane materials, were localized linearly along the margin of the laminal beams, pial septa and adventitia. They were also stained in all vascular walls. Collagen type V, VI and fibronectin were similar with collagen type I and III, but their linear staining was more intense onto the margin of the laminar beams and the pial septa. Rich immunoreactivity for alpha-elastin was found in the glial columns and the laminal beams. The presence and distribution of the structural protein components of the ECM of the monkey optic nerve lamina cribrosa was the same as that previously reported in human.
采用免疫组织化学方法——生物素-链霉亲和素（B-SA）系统，证实了正常猴视神经筛板中I型至VI型胶原蛋白、层粘连蛋白、纤连蛋白和α-弹性蛋白的存在及分布情况。I型和III型间质胶原在层束内呈弥漫性分布，并与周围巩膜连续。中央动静脉周围的外膜和软脑膜隔也被I型和III型胶原染色。作为基底膜物质的IV型胶原和层粘连蛋白，沿层束边缘、软脑膜隔和外膜呈线性分布。它们在所有血管壁中也有染色。V型、VI型胶原和纤连蛋白与I型和III型胶原情况相似，但它们在层束边缘和软脑膜隔上的线性染色更强。在神经胶质柱和层束中发现了丰富的α-弹性蛋白免疫反应性。猴视神经筛板细胞外基质结构蛋白成分的存在和分布与先前在人类中的报道相同。
