Fukuchi T, Sawaguchi S, Hara H, Shirakashi M, Iwata K
Department of Ophthalmology, Niigata University, School of Medicine, Japan.
Graefes Arch Clin Exp Ophthalmol. 1992;230(5):421-7. doi: 10.1007/BF00175926.
Using light microscopic immunohistochemistry, we studied the immunolocalization and immunoreactivity of the extracellular matrix, including collagen types III, IV, VI, laminin, and alpha elastin in the lamina cribrosa of monkey eyes with normal and experimentally chronic glaucoma. Our results showed: (1) abnormal linearlike immunodeposits of both collagen type IV and laminin in the margin of the lamina cribrosa with significant density in the glaucomatous eyes; (2) the immunoreactivity of collagen type III resembled that of the normal eye, but was slightly stronger at the laminar surface; (3) findings with collagen type VI resembled those of type III with an enhanced linearlike staining surrounding the nerve-fiber bundles. Furthermore, staining of alpha elastin demonstrated dramatic changes in both reactivity and localization. The lamina cribrosa of glaucomatous eyes showed a markedly reduced immunoreactivity as well as an irregular, interrupted pattern. These observations suggest that the changes might be a secondary to the long-standing elevation of intraocular pressure. The alteration of these macromolecules may modify the course of glaucomatous optic damage.
我们运用光学显微镜免疫组织化学方法，研究了正常及实验性慢性青光眼猴眼筛板中细胞外基质（包括III型、IV型、VI型胶原蛋白、层粘连蛋白和α弹性蛋白）的免疫定位和免疫反应性。我们的研究结果显示：（1）在青光眼眼中，IV型胶原蛋白和层粘连蛋白在筛板边缘出现异常的线性免疫沉积物，且密度显著；（2）III型胶原蛋白的免疫反应性与正常眼相似，但在板层表面稍强；（3）VI型胶原蛋白的结果与III型相似，在神经纤维束周围出现增强的线性染色。此外，α弹性蛋白的染色显示其反应性和定位都发生了显著变化。青光眼眼的筛板显示出免疫反应性明显降低以及不规则、中断的模式。这些观察结果表明，这些变化可能是眼压长期升高的继发结果。这些大分子的改变可能会改变青光眼性视神经损伤的进程。
