参与人类烧伤后瘢痕疙瘩形成的富含艾杜糖醛酸和富含葡萄糖醛酸的蛋白聚糖的纯化与特性分析

Purification and characterization of iduronic acid-rich and glucuronic acid-rich proteoglycans implicated in human post-burn keloid scar.

作者信息

Garg H G, Lippay E W, Burd D A, Neame P J

机构信息

Department of Biological Chemistry, and Molecular Pharmacology, Harvard Medical School, Shriners Burns Institute, Boston, Massachusetts.

出版信息

Carbohydr Res. 1990 Oct 25;207(2):295-305. doi: 10.1016/0008-6215(90)84056-z.

DOI:10.1016/0008-6215(90)84056-z

PMID:2076520

Abstract

Small proteoglycans (PGs), extracted from human keloid scar tissue with 4M guanidinium chloride and fractionated by DEAE-cellulose chromatography, were separated by ethanol precipitation into one L-iduronic acid-rich and one D-glucuronic acid-rich fraction. The size of the L-iduronic acid-rich PG was 102 kDa with a 27 kDa glycosaminoglycan chain, that of the D-glucuronic acid-rich PG was 90 kDa with a 26 kDa glycosaminoglycan chain, and the protein core of both PGs was 14.5 kDa. The two PGs carried sulfate groups mostly attached at C-4 of the 2-amino-2-deoxy-D-galactose units. The N-terminal amino acid sequence of both was similar to human bone PGII (decorin), normal and hypertrophic scar, and human dermal tissue PG.

摘要

从小鼠瘢痕疙瘩组织中用4M氯化胍提取并通过DEAE-纤维素色谱法分级分离的小蛋白聚糖（PGs），通过乙醇沉淀分离成一个富含L-艾杜糖醛酸的级分和一个富含D-葡萄糖醛酸的级分。富含L-艾杜糖醛酸的PG大小为102 kDa，糖胺聚糖链为27 kDa；富含D-葡萄糖醛酸的PG大小为90 kDa，糖胺聚糖链为26 kDa，两种PG的蛋白质核心均为14.5 kDa。这两种PG携带的硫酸基团大多连接在2-氨基-2-脱氧-D-半乳糖单元的C-4位。两者的N端氨基酸序列与人类骨PGII（饰胶蛋白聚糖）、正常和肥厚性瘢痕以及人类真皮组织PG相似。