Garg H G, Lippay E W, Burd D A, Neame P J
Department of Biological Chemistry, and Molecular Pharmacology, Harvard Medical School, Shriners Burns Institute, Boston, Massachusetts.
Carbohydr Res. 1990 Oct 25;207(2):295-305. doi: 10.1016/0008-6215(90)84056-z.
Small proteoglycans (PGs), extracted from human keloid scar tissue with 4M guanidinium chloride and fractionated by DEAE-cellulose chromatography, were separated by ethanol precipitation into one L-iduronic acid-rich and one D-glucuronic acid-rich fraction. The size of the L-iduronic acid-rich PG was 102 kDa with a 27 kDa glycosaminoglycan chain, that of the D-glucuronic acid-rich PG was 90 kDa with a 26 kDa glycosaminoglycan chain, and the protein core of both PGs was 14.5 kDa. The two PGs carried sulfate groups mostly attached at C-4 of the 2-amino-2-deoxy-D-galactose units. The N-terminal amino acid sequence of both was similar to human bone PGII (decorin), normal and hypertrophic scar, and human dermal tissue PG.
从小鼠瘢痕疙瘩组织中用4M氯化胍提取并通过DEAE-纤维素色谱法分级分离的小蛋白聚糖（PGs），通过乙醇沉淀分离成一个富含L-艾杜糖醛酸的级分和一个富含D-葡萄糖醛酸的级分。富含L-艾杜糖醛酸的PG大小为102 kDa，糖胺聚糖链为27 kDa；富含D-葡萄糖醛酸的PG大小为90 kDa，糖胺聚糖链为26 kDa，两种PG的蛋白质核心均为14.5 kDa。这两种PG携带的硫酸基团大多连接在2-氨基-2-脱氧-D-半乳糖单元的C-4位。两者的N端氨基酸序列与人类骨PGII（饰胶蛋白聚糖）、正常和肥厚性瘢痕以及人类真皮组织PG相似。
