Inoue S, Iwasaki M
J Biochem. 1976 Sep;80(3):513-24. doi: 10.1093/oxfordjournals.jbchem.a131306.
Dermatan sulfate-chondroitin sulfate copolymers have been isolated from human umbilical cord as a major galactosaminoglycan component of this tissue. The galactosaminoglycan fraction was obtained from this tissue by papain [EC 3.4.22.2] digestion followed by precipitation with cetylpyridinium chloride in a yield of 700 mg per 100 g of dry tissue. Ethanol fractionation resolved 4-5 subfractions differing in relative content of L-iduronic acid and D-glucuronic acid. No galactosaminoglycan containing either solely L-iduronic acid or D-glucuronic acid was obtained. The copolymeric structure of the material in each subfraction was demonstrated by analysis of oligosaccharide fragments obtained by chondroitinase-AC [EC 4.2.2.5] digestion. All the polymers contained repeating disaccharide units, D-glucuronosyl-N-acetylgalactosamine, D-glucuronosyl-N-acetylgalactosamine 4-sulfate, D-glucuronosyl-N-acetyl-galactosamine 6-sulfate, and L-iduronosyl-N-acetylgalactosamine 4-sulfate, of which D-glucuronosyl-N-acetylgalactosamine 6-sulfate and L-iduronosyl-N-acetylgalactosamine 4-sulfate were predominant. Both iduronic acid- and glucuronic acid-containing units were arranged in clusters. The presence of a considerable amount of nonsulfated disaccharide units was noted. The copolymers show extensive polydispersity in electrophoresis on cellulose acetate and gel chromatography on Sephadex G-200.
硫酸皮肤素-硫酸软骨素共聚物已从人脐带中分离出来，作为该组织主要的氨基半乳糖聚糖成分。通过木瓜蛋白酶[EC 3.4.22.2]消化该组织，随后用十六烷基氯化吡啶沉淀，从该组织中获得氨基半乳糖聚糖部分，每100克干组织的产量为700毫克。乙醇分级分离得到4 - 5个亚级分，它们在L-艾杜糖醛酸和D-葡萄糖醛酸的相对含量上有所不同。未获得仅含有L-艾杜糖醛酸或D-葡萄糖醛酸的氨基半乳糖聚糖。通过分析软骨素酶-AC[EC 4.2.2.5]消化得到的寡糖片段，证实了每个亚级分中物质的共聚物结构。所有聚合物都含有重复的二糖单元，D-葡萄糖醛酸基-N-乙酰半乳糖胺、D-葡萄糖醛酸基-N-乙酰半乳糖胺4-硫酸盐、D-葡萄糖醛酸基-N-乙酰半乳糖胺6-硫酸盐和L-艾杜糖醛酸基-N-乙酰半乳糖胺4-硫酸盐，其中D-葡萄糖醛酸基-N-乙酰半乳糖胺6-硫酸盐和L-艾杜糖醛酸基-N-乙酰半乳糖胺4-硫酸盐占主导。含艾杜糖醛酸和葡萄糖醛酸的单元都成簇排列。注意到存在相当数量的非硫酸化二糖单元。这些共聚物在醋酸纤维素电泳和Sephadex G-200凝胶色谱中表现出广泛的多分散性。
