Gupta R, Yewdell J W, Olmsted R A, Collins P L, Bennink J R
Wistar Institute, Philadelphia, Pennsylvania.
Microb Pathog. 1990 Jul;9(1):13-8. doi: 10.1016/0882-4010(90)90036-p.
Effector cells capable of lysing respiratory syncytial virus (RSV)-infected cells were isolated from the lungs of intranasally infected mice. An examination of their specificity showed that cytolysis was major histocompatibility complex restricted. Using recombinant vaccinia viruses containing cloned RSV genes to infect target cells, BALB/c (H-2d) pulmonary effector cells were shown to recognize the fusion protein (F) and to a lesser extent the nucleoprotein (N). Cells specific for the major glycoprotein (G) or the small hydrophobic protein (1A) were not observed.
从经鼻感染小鼠的肺中分离出能够裂解呼吸道合胞病毒（RSV）感染细胞的效应细胞。对其特异性的检测表明，细胞溶解受主要组织相容性复合体限制。使用含有克隆的RSV基因的重组痘苗病毒感染靶细胞，结果显示BALB/c（H-2d）肺效应细胞可识别融合蛋白（F），对核蛋白（N）的识别程度较低。未观察到对主要糖蛋白（G）或小疏水蛋白（1A）具有特异性的细胞。
