Sharma A K, Woldehiwet Z
Department of Veterinary Pathology, University of Liverpool, Neston, UK.
Clin Exp Immunol. 1995 Aug;101(2):288-94. doi: 10.1111/j.1365-2249.1995.tb08353.x.
The lamb is a good model to study the pathogenesis and immune responses to infections with respiratory syncytial virus (RSV) as lambs experimentally infected with bovine or human RSV may develop overt clinical disease. In the present study the development of cellular cytotoxic responses was studied in splenic, pulmonary and peripheral blood mononuclear cells obtained from lambs after primary and secondary infection with bovine RSV. Infection with bovine RSV was followed by the appearance of cytotoxic cells in the peripheral blood, the spleen and lung lavage fluids. These effector cells lysed virus-infected targets in a self-restricted manner. Depletion techniques revealed that cytotoxic activity was largely due to OvCD8+ cells. When effector cells obtained from primed lambs were stimulated with inactivated bovine RSV or with virus-infected cells in vitro, virus-specific cytotoxicity was significantly increased.
羔羊是研究呼吸道合胞病毒（RSV）感染的发病机制和免疫反应的良好模型，因为经牛或人RSV实验感染的羔羊可能会出现明显的临床疾病。在本研究中，对初次和二次感染牛RSV后的羔羊所获得的脾、肺和外周血单核细胞中的细胞毒性反应的发展进行了研究。感染牛RSV后，外周血、脾脏和肺灌洗液中出现了细胞毒性细胞。这些效应细胞以自我限制的方式裂解病毒感染的靶标。去除技术显示，细胞毒性活性主要归因于OvCD8+细胞。当用灭活的牛RSV或体外病毒感染的细胞刺激从致敏羔羊获得的效应细胞时，病毒特异性细胞毒性显著增加。
