人淋巴细胞和成淋巴细胞中的环磷酸腺苷磷酸二酯酶。
Cyclic AMP phosphodiesterase in human lymphocytes and lymphoblasts.
作者信息
Takemoto D J, Lee W N, Kaplan S A, Appleman M M
出版信息
J Cyclic Nucleotide Res. 1978 Apr;4(2):123-32.
Cyclic nucleotide phosphodiesterase activities were examined in lymphocytes from 12 transformed human B cell lines, two T cell lines, six patients with lymphocytic leukemia, and 10 normal donors. A consistent difference bwtween cells from the normal and leukemic state was observed. The cyclic AMP phosphodiesterase activity from normal lymphocytes is inhibited greater than 80% by muM cyclic GMP while this concentration of nucleotide has little or no effect on the enzyme from transformed lymphocytic cell lines or from lymphocytic cells of leukemia patients. The reported lack of cyclic GMP phosphodiesterase in human lymphocytes from several sources is confirmed. The apparent absence of a cyclic GMP degradation mechanism and of cyclic GMP control of cyclic AMP hydrolysis may be related to defective lymphocyte growth control.
对来自12个人类转化B细胞系、2个T细胞系、6例淋巴细胞白血病患者及10名正常供者的淋巴细胞中的环核苷酸磷酸二酯酶活性进行了检测。观察到正常状态与白血病状态的细胞之间存在一致的差异。正常淋巴细胞的环磷酸腺苷磷酸二酯酶活性被微摩尔级的环磷酸鸟苷抑制超过80%，而该核苷酸浓度对转化淋巴细胞系或白血病患者淋巴细胞中的该酶几乎没有影响。证实了来自多个来源的人类淋巴细胞中缺乏环磷酸鸟苷磷酸二酯酶的报道。环磷酸鸟苷降解机制的明显缺失以及环磷酸鸟苷对环磷酸腺苷水解的控制缺失可能与淋巴细胞生长控制缺陷有关。
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