Ortez R A, Hersh E M, Robison G A
J Cyclic Nucleotide Res. 1978 Apr;4(2):87-96.
Cyclic AMP dependent protein kinase activity was depressed in whole thymus and spleen as well as isolated splenic lymphocytes from B16 melanoma bearing C57B1/6J mice as compared to control animals. A similar loss of enzyme activity was observed in human peripheral blood lymphocytes from melanoma bearing patients as compared to normal subjects. An unaltered level of activity in the heart of tumor bearing mice suggested some specificity for the lymphoid system. This depressed enzyme activity was the result of a diminished Vmax for cAMP stimulated calf histone phosphorylation. The tumor bearing state in the mouse was also accompanied by a depletion of small lymphocytes from both thymus and spleen and it is hypothesized that the losses of lymphocytes and cAMP dependent protein kinase activity are related.
与对照动物相比，携带B16黑色素瘤的C57B1/6J小鼠的整个胸腺、脾脏以及分离出的脾淋巴细胞中的环磷酸腺苷依赖性蛋白激酶活性均降低。与正常受试者相比，黑色素瘤患者的人外周血淋巴细胞中也观察到类似的酶活性丧失。荷瘤小鼠心脏中的活性水平未改变，提示对淋巴系统具有一定特异性。这种酶活性降低是由于环磷酸腺苷刺激的小牛组蛋白磷酸化的最大反应速度降低所致。小鼠的荷瘤状态还伴随着胸腺和脾脏中小淋巴细胞的减少，据推测，淋巴细胞的减少与环磷酸腺苷依赖性蛋白激酶活性的降低有关。
