Michel P, Michault A, Gruel J C, Coulanges P
Institut Pasteur, Tananarive, Madagascar.
Arch Inst Pasteur Madagascar. 1990;57(1):115-42.
Cysticercosis is linked to the infestation of men (or pigs) by the larval stage of Toenia solium: Cysticercus cellulosae. This disease, very common in the Third World, represents in Madagascar, a very serious problem for Public Health. Neurocysticercosis, particularly, must be detected before the stage of evolution towards cicatrisation. Two biological tests have been developed: 1) ELISA test: Cysticercus antigen is prepared from pig cysticercus, with addition of PMSF: proteases inhibitor increasing antigen stability. We present sensitivity and specificity studies of the test. 2) Western Blot test: E.I.T.B.: this test completes first test in case of negative ELISA test with clinical signs. In this case, evolving diagnosis is supported by low weight proteins revelation by patient's serum. These two tests applied to 100 positives cases have permitted the chemotherapy indication, with favorable results in more that 80% of the cases.
囊尾蚴病与猪带绦虫幼虫期（猪囊尾蚴）感染人体（或猪）有关。这种疾病在第三世界非常常见，在马达加斯加，它对公共卫生来说是一个非常严重的问题。特别是神经囊尾蚴病，必须在向瘢痕化演变阶段之前被检测出来。已经开发了两种生物学检测方法：1）酶联免疫吸附测定（ELISA）试验：猪囊尾蚴抗原由猪囊尾蚴制备，并添加苯甲基磺酰氟（PMSF）：一种蛋白酶抑制剂，可提高抗原稳定性。我们展示了该检测方法的敏感性和特异性研究。2）蛋白质印迹试验：酶免疫转印印迹法（E.I.T.B.）：如果ELISA试验呈阴性但有临床症状，该检测方法可完善初次检测。在这种情况下，通过患者血清中低分子量蛋白质的显示来辅助进行逐步诊断。这两种检测方法应用于100例阳性病例，为化疗提供了依据，超过80%的病例取得了良好效果。
