Lenoir M C, Bernard B A
Cell Biology Department, Centre International de Recherches Dermatologiques Galderma, Valbonne, France.
Skin Pharmacol. 1990;3(2):97-106. doi: 10.1159/000210856.
Epidermis was obtained in vitro after air exposure of keratinocyte cultures grown on a dermal equivalent. Some cultures were established from enzymatically dissociated keratinocytes of either interfollicular epidermis or hair follicle outer root sheath. Others resulted from centrifugal outgrowth of epidermal sheet, out of skin biopsies or hair follicles, which were directly implanted into dermal equivalents. Whatever the system used, a multilayered epidermis was obtained with an overall architecture resembling that of human epidermis. However, depending on the tissue culture method used and the source of keratinocytes, significant differences were observed. The most striking finding was the difference in 67 kDa keratin expression: the only case where it was strictly suprabasal and homogeneously expressed in the cytoplasm of the cells, as in normal epidermis, was found in the epidermis obtained from hair follicle explants. With the other methods, the expression of this marker was delayed and patchy. These results are discussed in terms of possible intrinsic differences between interfollicular and follicular keratinocytes.
在真皮替代物上培养的角质形成细胞暴露于空气中后，在体外获取表皮。一些培养物是从毛囊间表皮或毛囊外根鞘的酶解角质形成细胞建立的。其他培养物则来自于从皮肤活检组织或毛囊中离心生长出的表皮片，这些组织被直接植入真皮替代物中。无论使用何种系统，都能获得具有类似于人类表皮整体结构的多层表皮。然而，根据所使用的组织培养方法和角质形成细胞的来源，观察到了显著差异。最显著的发现是67 kDa角蛋白表达的差异：唯一在细胞胞质中严格位于基底层以上且均匀表达的情况，如同正常表皮一样，见于从毛囊外植体获得的表皮。使用其他方法时，该标志物的表达延迟且呈斑片状。根据毛囊间和毛囊角质形成细胞之间可能存在的内在差异对这些结果进行了讨论。
