Rodnight R, Leal R
Departamento de Bioquímica, Universidade Federal do Rio Grande do Sul (Centro), Porto Alegre, Brazil.
J Mol Neurosci. 1990;2(2):115-22. doi: 10.1007/BF02876919.
Regional variations in protein phosphorylating activity in the rat brain were studied. Micro-slices (1 mm diameter) were prepared from 19 brain areas, phosphoproteins labeled by incubation with [32P]phosphate, and the tissue analyzed by nonequilibrium two-dimensional electrophoresis and autoradiography. Attention was focused on three phosphorylating systems that showed consistent variation in activity. (1) A system that phosphorylates a substrate of 47 kDa (ppH-47) whose activity was highest in the hippocampus. The next highest activity of this system was observed in the globus pallidus, followed by the periventricular gray matter of the aqueduct, lateral septum, cerebellar cortex, entorhinal cortex, hypothalamus, mammillary nuclei, amygdala, and substantia nigra. Activity was low or undetectable in the cerebral cortex, neostriatum, and the colliculi. (2) A system that phosphorylates a substrate of 50 kDa (ppC-50) whose activity was highest in the caudate nucleus. The activity of this system was roughly inversely correlated with that of the ppH-47 system. (3) The protein kinase C system that phosphorylates an 82- to 87-kDa substrate known as MARCKS. The highest activity of this system was observed in the cerebellar cortex, followed by the hypothalamus, mammillary nuclei, periventricular gray matter of the aqueduct, and the superior colliculus. Activity of this system was relatively low in several regions of the cerebral cortex, the neostriatum, and the inferior colliculus.
对大鼠脑中蛋白质磷酸化活性的区域差异进行了研究。从19个脑区制备了微切片（直径1毫米），通过与[32P]磷酸盐孵育标记磷蛋白，然后用非平衡二维电泳和放射自显影对组织进行分析。重点关注了三个活性呈现一致变化的磷酸化系统。（1）一个磷酸化47 kDa底物（ppH - 47）的系统，其活性在海马体中最高。该系统的次高活性在苍白球中观察到，其次是导水管周围灰质、外侧隔、小脑皮质、内嗅皮质、下丘脑、乳头体核、杏仁核和黑质。在大脑皮质、新纹状体和丘脑中活性较低或无法检测到。（2）一个磷酸化50 kDa底物（ppC - 50）的系统，其活性在尾状核中最高。该系统的活性与ppH - 47系统的活性大致呈负相关。（3）蛋白质激酶C系统，其磷酸化一种称为MARCKS的82至87 kDa底物。该系统的最高活性在小脑皮质中观察到，其次是下丘脑、乳头体核、导水管周围灰质和上丘。该系统的活性在大脑皮质的几个区域、新纹状体和下丘中相对较低。
