Rodnight R, Zamani R, Tweedale A
Department of Biochemistry, Institute of Psychiatry, London, U.K.
J Neurosci Methods. 1988 May;24(1):27-38. doi: 10.1016/0165-0270(88)90030-1.
Procedures are described for studying protein phosphorylation in 1 mm diameter micro-slices of rat brain tissue using two-dimensional electrophoresis as analytical tool. The activity of several protein phosphorylating systems, including a major system phosphorylating a 40 kDa substrate complex, was highly dependent on the procedures used for micro-slice preparation and on the Ca2+-content of the preparation medium. Under optimal conditions the pattern of phosphorylation observed in micro-slices closely resembled that obtained by in vivo labelling.
描述了使用二维电泳作为分析工具研究大鼠脑组织1毫米直径微切片中蛋白质磷酸化的方法。几种蛋白质磷酸化系统的活性，包括磷酸化一种40 kDa底物复合物的主要系统，高度依赖于用于微切片制备的方法以及制备培养基中的Ca2+含量。在最佳条件下，微切片中观察到的磷酸化模式与体内标记获得的模式非常相似。
