腺病毒诱导的细胞脱氧核糖核酸酶抑制
Adenovirus-induced inhibition of cellular DNase.
作者信息
Nass K, Frenkel G D
出版信息
J Virol. 1978 May;26(2):540-3. doi: 10.1128/JVI.26.2.540-543.1978.
Abstract
During the productive infection of KB cells by adenovirus type 5 (Ad5), there is a progressive decrease in the level of cellular DNase activity towards single-stranded DNA, in contrast to DNA polymerase which remains relatively constant throughout the infection. This decrease is prevented by the inhibition of protein synthesis by cycloheximide. The inhibition of DNase activity does not occur after infection by Ad5 ts125, a DNA-negative mutant which fails to induce the adenovirus-specific DNA binding protein. In contrast, infection by Ad5 ts36, a DNA-negative mutant which complements ts125, does result in decreased levels of DNase. A mechanism is discussed in which the DNA binding protein protects viral replicative intermediates from degradation by cellular DNase.
摘要
在5型腺病毒（Ad5）对KB细胞的增殖性感染过程中，细胞对单链DNA的脱氧核糖核酸酶活性水平逐渐下降，而DNA聚合酶在整个感染过程中保持相对恒定。用环己酰亚胺抑制蛋白质合成可阻止这种下降。Ad5 ts125（一种DNA阴性突变体，不能诱导腺病毒特异性DNA结合蛋白）感染后，脱氧核糖核酸酶活性不会受到抑制。相反，Ad5 ts36（一种能互补ts125的DNA阴性突变体）感染确实会导致脱氧核糖核酸酶水平下降。文中讨论了一种机制，即DNA结合蛋白可保护病毒复制中间体不被细胞脱氧核糖核酸酶降解。
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