腺病毒DNA结合蛋白对DNase的抑制作用表现出对该酶的特异性，而非对DNA二级结构的特异性。

DNase inhibition by the adenovirus DNA-binding protein exhibits specificity for the enzyme but not for the secondary structure of the DNA.

作者信息

Frenkel G D, Horan K

出版信息

Biochem Biophys Res Commun. 1983 Jan 27;110(2):443-8. doi: 10.1016/0006-291x(83)91169-5.

Abstract

The adenovirus-specific DNA-binding protein (DBP) has been shown to inhibit the hydrolysis of single-stranded DNA by a DNase isolated from KB cells, (Nass, K., and Frenkel, G.D. (1980). J. Virol. 35, 314-319). The specificity of the inhibition has now been investigated. The DBP inhibits the hydrolysis of single-stranded DNA by several different DNases (DNase II, KB DNase, S1 nuclease) under a variety of reaction conditions, but it has no effect on DNase I-catalyzed hydrolysis of single-stranded DNA. The DBP also inhibits the rate of hydrolysis of double-stranded DNA by KB DNase and DNase II, but has no effect on DNase I-catalyzed hydrolysis of this substrate. The DBP also inhibits the dephosphorylation of 5'-phosphoryl-terminated DNA by bacterial alkaline phosphatase but stimulates the phosphorylation of 5'-hydroxyl-terminated DNA by polynucleotide kinase.

摘要

腺病毒特异性DNA结合蛋白（DBP）已被证明可抑制从KB细胞中分离出的一种脱氧核糖核酸酶对单链DNA的水解作用（纳斯，K.，和弗伦克尔，G.D.（1980年）。《病毒学杂志》35卷，314 - 319页）。现在已经对这种抑制作用的特异性进行了研究。在多种反应条件下，DBP可抑制几种不同的脱氧核糖核酸酶（脱氧核糖核酸酶II、KB脱氧核糖核酸酶、S1核酸酶）对单链DNA的水解，但对脱氧核糖核酸酶I催化的单链DNA水解没有影响。DBP还可抑制KB脱氧核糖核酸酶和脱氧核糖核酸酶II对双链DNA的水解速率，但对脱氧核糖核酸酶I催化的该底物水解没有影响。DBP还可抑制细菌碱性磷酸酶对5'-磷酸末端DNA的去磷酸化作用，但可刺激多核苷酸激酶对5'-羟基末端DNA的磷酸化作用。