Slavícková A
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Praha.
Electrophoresis. 1990 Oct;11(10):892. doi: 10.1002/elps.1150111020.
A gel matrix composed of 0.5% agarose and 2.5% polyacrylamide allows the parallel electrophoretic separation of nonreduced IgM and IgG monoclonal antibodies. The mobilities of IgM and IgG differ at pH 9.5, which enables a preliminary Ig class designation. It allows rapid detection of incomplete cloning if both IgM and IgG are present. A semiquantitative evaluation of nonreduced IgM in ascitic fluid is possible as well.
由0.5%琼脂糖和2.5%聚丙烯酰胺组成的凝胶基质可对非还原型IgM和IgG单克隆抗体进行平行电泳分离。在pH 9.5时，IgM和IgG的迁移率不同，这使得可以初步确定Ig类别。如果同时存在IgM和IgG，则能够快速检测出克隆不完全的情况。对腹水中非还原型IgM进行半定量评估也是可行的。
