单克隆免疫球蛋白定量方法的比较。
A comparison of methods of monoclonal immunoglobulin quantitation.
作者信息
Tichý M
出版信息
Neoplasma. 1985;32(1):31-6.
Quantitation of monoclonal immunoglobulins was carried out using the following methods: densitometric scanning of electrophoretograms on cellulose acetate gel, single radial immunodiffusion, nephelometry and, in monoclonal IgM, also sedimentation analysis. To compare the methods, 20 sera with monoclonal IgG, 16 sera with monoclonal IgA and 19 sera with monoclonal IgM were used. The methods correlated well in monoclonal IgM and IgG while in IgA correlation was found only between densitometry and radial immunodiffusion. Closest correlation was observed between densitometry and sedimentation analysis in quantifying monoclonal IgM (r = 0.954). Despite good correlation, the individual values of monoclonal immunoglobulins established by different methods showed marked differences. In comparison with the immunochemical methods, the quantitation of monoclonal immunoglobulins using densitometric scanning of electrophoretograms is accessible, quick and relatively more precise method.
采用以下方法对单克隆免疫球蛋白进行定量
对醋酸纤维素凝胶上的电泳图谱进行光密度扫描、单向放射免疫扩散、散射比浊法，对于单克隆IgM，还采用沉降分析。为比较这些方法，使用了20份含单克隆IgG的血清、16份含单克隆IgA的血清和19份含单克隆IgM的血清。这些方法在单克隆IgM和IgG中相关性良好，而在IgA中仅在光密度测定法和放射免疫扩散之间发现相关性。在定量单克隆IgM时，光密度测定法和沉降分析之间观察到最密切的相关性（r = 0.954）。尽管相关性良好，但不同方法确定的单克隆免疫球蛋白的个体值显示出明显差异。与免疫化学方法相比，利用电泳图谱光密度扫描对单克隆免疫球蛋白进行定量是一种可行、快速且相对更精确的方法。
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