通过固相萃取和半制备高效液相色谱法分离长春花生物碱。
Isolation of Catharanthus alkaloids by solid-phase extraction and semipreparative HPLC.
作者信息
Naaranlahti T, Nordström M, Lapinjoki S P, Huhtikangas A, Lounasmaa M
机构信息
Department of Pharmaceutical Chemistry, University of Kuopio, Finland.
出版信息
J Chromatogr Sci. 1990 Apr;28(4):173-4. doi: 10.1093/chromsci/28.4.173.
PMID:2079546
Abstract
A combination of moderate-pressure chromatography on C18 sorbent and preparative HPLC is developed for rapid isolation of alkaloids from Catharanthus roseus. The procedure is optimized for vindoline and catharanthine with respective yields of 3 and 2 mg per 1 g of dried leaves of the plant. The methodology is also applied for identification of the above and other alkaloids from cultured plant cells.
摘要
开发了一种结合C18吸附剂上的中压色谱法和制备型高效液相色谱法，用于从长春花中快速分离生物碱。该方法针对文多灵和长春质碱进行了优化，从每1克植物干叶中分别获得3毫克和2毫克的产量。该方法还用于从培养的植物细胞中鉴定上述生物碱和其他生物碱。
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