Engineering Research Center of Forestry Bio-preparation, Ministry of Education, Northeast Forestry University, Harbin 150040, China.
Molecules. 2012 Jul 25;17(8):8742-52. doi: 10.3390/molecules17088742.
In the present study, an improved method termed negative-pressure cavitation extraction (NPCE) followed by reverse phase high-performance liquid chromatography (RP-HPLC) was developed for the extraction and quantification of vindoline (VDL), catharanthine (CTR), vincristine (VCR) and vinblastine (VLB) from Catharanthus roseus leaves. The optimized method employed 60-mesh particles, 80% ethanol, a negative pressure of -0.075 MPa, a solid to liquid ratio of 1:20, 30 min of extraction and three extraction cycles. Under these optimized conditions, the extraction yields of VDL, CTR, VCR and VLB are 0.5783, 0.2843, 0.018 and 0.126 mg/g DW, respectively. These extraction yields are equivalent to those from the well-known ultrasonic extraction method and higher than the yields from maceration extraction and heating reflux extraction. Our results suggest that NPCE-RP-HPLC represents an excellent alternative for the extraction and quantification of vinca alkaloids for pilot- and industrial-scale applications.
在本研究中，开发了一种改良的方法，称为负压空化提取（NPCE），随后进行反相高效液相色谱（RP-HPLC），用于从长春花叶中提取和定量 vindoline（VDL）、长春质碱（CTR）、长春新碱（VCR）和长春碱（VLB）。优化后的方法采用 60 目颗粒、80%乙醇、-0.075 MPa 的负压、固液比为 1:20、提取 30 分钟和三个提取循环。在这些优化条件下，VDL、CTR、VCR 和 VLB 的提取收率分别为 0.5783、0.2843、0.018 和 0.126 mg/g DW。这些提取收率与著名的超声提取方法相当，高于浸渍提取和加热回流提取的收率。我们的结果表明，NPCE-RP-HPLC 代表了一种用于提取和定量长春烷生物碱的优秀替代方法，适用于中试和工业规模应用。
