Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
J Proteomics. 2010 Oct 10;73(11):2078-91. doi: 10.1016/j.jprot.2010.08.005. Epub 2010 Aug 23.
The emergence of shotgun proteomics has facilitated the numerous biological discoveries made by proteomic studies. However, comprehensive proteomic analysis remains challenging and shotgun proteomics is a continually changing field. This review details the recent developments in shotgun proteomics and describes emerging technologies that will influence shotgun proteomics going forward. In addition, proteomic studies of integral membrane proteins remain challenging due to the hydrophobic nature in integral membrane proteins and their general low abundance levels. However, there have been many strategies developed for enriching, isolating and separating membrane proteins for proteomic analysis that have moved this field forward. In summary, while shotgun proteomics is a widely used and mature technology, the continued pace of improvements in mass spectrometry and proteomic technology and methods indicate that future studies will have an even greater impact on biological discovery.
shotgun 蛋白质组学的出现促进了蛋白质组学研究取得的众多生物学发现。然而，全面的蛋白质组学分析仍然具有挑战性，shotgun 蛋白质组学是一个不断变化的领域。本文详细介绍了 shotgun 蛋白质组学的最新进展，并描述了将影响未来 shotgun 蛋白质组学发展的新兴技术。此外，由于整合膜蛋白的疏水性和一般低丰度水平，整合膜蛋白的蛋白质组学研究仍然具有挑战性。然而，已经开发出许多用于蛋白质组学分析的富集、分离和分离膜蛋白的策略，推动了该领域的发展。总之，虽然 shotgun 蛋白质组学是一种广泛使用且成熟的技术，但质谱和蛋白质组学技术和方法的持续改进步伐表明，未来的研究将对生物发现产生更大的影响。
Zotero 插件