Biotechnology Division, Biomedical Research Foundation, Academy of Athens, Greece Soranou Efessiou 4,11527, Athens, Greece.
Expert Rev Mol Diagn. 2014 Jun;14(5):549-63. doi: 10.1586/14737159.2014.917965. Epub 2014 May 12.
Membrane proteins are involved in central processes such as cell signaling, cell-cell interactions and communication, ion and metabolite transport and in general play a crucial role in cell homeostasis. Cancer and cancer metastasis have been correlated to protein expression levels and dysfunction, with membrane proteins playing an important role, and are thus used as drug targets and potential biomarkers for prognostic or diagnostic purposes. Despite the critical biological significance of membrane proteins, proteomic analysis has been a challenging task due to their hydrophobicity. In this review, recent advances in the proteomic analysis of membrane proteins are presented, focusing on membrane fraction enrichment techniques combined with labeled or label-free shotgun proteomics approaches for the identification of potential cancer biomarkers.
膜蛋白参与细胞信号转导、细胞间相互作用和通讯、离子和代谢物运输等核心过程，在细胞稳态中起着至关重要的作用。癌症和癌症转移与蛋白质表达水平和功能障碍有关，膜蛋白起着重要作用，因此被用作药物靶点和潜在的预后或诊断标志物。尽管膜蛋白具有重要的生物学意义，但由于其疏水性，蛋白质组学分析一直是一项具有挑战性的任务。本文综述了膜蛋白蛋白质组学分析的最新进展，重点介绍了膜蛋白分离技术与标记或非标记的鸟枪法蛋白质组学方法相结合，用于鉴定潜在的癌症生物标志物。
