Teruel M, Sørensen J G, Loeschcke V, Cabrero J, Perfectti F, Camacho J P M
Departamento de Genética, Universidad de Granada, Granada, Spain.
Cytogenet Genome Res. 2011;132(1-2):94-9. doi: 10.1159/000319621. Epub 2010 Aug 25.
We analyzed the effect of B-chromosome presence on expression level of heat shock protein 70 (Hsp70) in cerebral ganglion and gonad in both males and females of the grasshopper Eyprepocnemis plorans. Two natural Spanish populations, Salobreña (Granada) and Torrox (Málaga) were assayed, the former harbouring a neutralized (non-driving) B-chromosome (B(2)) and the latter a parasitic (driving) B-chromosome (B(24)). The analysis was performed by Western blotting, immunostaining and densitometric measuring expression level of the Hsp70 family in adult individuals. The results showed that Hsp70 levels of testis were significantly higher in Salobreña than Torrox, and were significantly lower in testes of B-carrying males from both populations. A similar effect was observed in the ovary of females from Torrox. No effect was, however, observed in cerebral ganglia in any sex or population. B-chromosome effects in Torrox showed a dose-dependent pattern. The results point to an interesting interaction between B-chromosome and stress protein expression in reproductive tissue.
我们分析了B染色体的存在对草蜢Eyprepocnemis plorans雄性和雌性个体脑神经节及性腺中热休克蛋白70（Hsp70）表达水平的影响。对两个西班牙自然种群进行了测定，一个是位于格拉纳达的萨洛布雷尼亚种群，另一个是位于马拉加的托罗克斯种群，前者含有一条中和型（非驱动型）B染色体（B(2)），后者含有一条寄生型（驱动型）B染色体（B(24)）。通过蛋白质免疫印迹法、免疫染色以及光密度法对成年个体中Hsp70家族的表达水平进行了分析。结果表明，萨洛布雷尼亚种群睾丸中的Hsp70水平显著高于托罗克斯种群，且两个种群中携带B染色体的雄性个体睾丸中的Hsp70水平显著更低。在托罗克斯种群雌性个体的卵巢中也观察到了类似的效应。然而，在任何性别或种群的脑神经节中均未观察到影响。托罗克斯种群中的B染色体效应呈现出剂量依赖性模式。结果表明B染色体与生殖组织中应激蛋白表达之间存在有趣的相互作用。
