Department of Biological Engineering, Seokyeong University, 16-1 Jungneung-dong, Sungbuk-gu, Seoul, Korea.
J Microbiol Biotechnol. 2010 Aug;20(8):1230-9. doi: 10.4014/jmb.0910.10016.
Five bacterial species, capable of degrading the recalcitrant organic compounds (ROCs) diethyleneglycol monomethylether (DGMME), 1-amino-2-propanol (APOL), 1-methyl-2- pyrrolidinone (NMP), diethyleneglycol monoethylether (DGMEE), tetraethyleneglycol (TEG), and tetrahydrothiophene 1,1-dioxide (sulfolane), were isolated from an enrichment culture. Cupriavidus sp. catabolized 93.5+/-1.7 mg/l of TEG, 99.3+/-1.2 mg/l of DGMME, 96.1+/-1.6mg/l of APOL, and 99.5+/-0.5mg/l of NMP in 3 days. Acineobacter sp. catabolized 100 mg/l of DGMME, 99.9+/-0.1 mg/l of NMP, and 100 mg/l of DGMEE in 3 days. Pseudomonas sp.3 catabolized 95.7+/-1.2 mg/l of APOL and 99.8+/-0.3 mg/l of NMP. Paracoccus sp. catabolized 98.3+/-0.6 mg/l of DGMME and 98.3+/-1.0 mg/l of DGMEE in 3 days. A maximum 43+/-2.0 mg/l of sulfolane was catabolized by Paracoccus sp. in 3 days. When a mixed culture composed of the five bacterial species was applied to real wastewater containing DGMME, APOL, NMP, DGMEE, or TEG, 92~99% of each individual ROC was catabolized within 3 days. However, at least 9 days were required for the complete mineralization of sulfolane. Bacterial community diversity, analyzed on the basis of the TGGE pattern of 16S rDNA extracted from viable cells, was found to be significantly reduced in a conventional bioreactor after 6 days of incubation. However, biodiversity was maintained after 12 days of incubation in an electrochemical bioreactor. In conclusion, the electrochemical reduction reaction enhanced the diversity of the bacterial community and actively catabolized sulfolane.
从富集培养物中分离出了 5 种能够降解难降解有机化合物（ROCs）二甘醇单甲醚（DGMME）、1-氨基-2-丙醇（APOL）、1-甲基-2-吡咯烷酮（NMP）、二甘醇单乙醚（DGMEE）、四甘醇（TEG）和四氢噻吩 1,1-二氧化物（环丁砜）的细菌。铜绿假单胞菌在 3 天内代谢了 93.5+/-1.7mg/l 的 TEG、99.3+/-1.2mg/l 的 DGMME、96.1+/-1.6mg/l 的 APOL 和 99.5+/-0.5mg/l 的 NMP。不动杆菌在 3 天内代谢了 100mg/l 的 DGMME、99.9+/-0.1mg/l 的 NMP 和 100mg/l 的 DGMEE。假单胞菌 3 代谢了 95.7+/-1.2mg/l 的 APOL 和 99.8+/-0.3mg/l 的 NMP。副球菌在 3 天内代谢了 98.3+/-0.6mg/l 的 DGMME 和 98.3+/-1.0mg/l 的 DGMEE。副球菌在 3 天内代谢了最多 43+/-2.0mg/l 的环丁砜。当将由 5 种细菌组成的混合培养物应用于含有 DGMME、APOL、NMP、DGMEE 或 TEG 的实际废水中时，在 3 天内每种 ROC 的转化率达到 92~99%。然而，环丁砜的完全矿化至少需要 9 天。基于从活细胞中提取的 16S rDNA 的 TGGE 图谱分析，发现经过 6 天的孵育，在传统生物反应器中细菌群落的多样性显著降低。然而，在电化学生物反应器中孵育 12 天后，生物多样性得到了维持。总之，电化学还原反应增强了细菌群落的多样性，并积极代谢了环丁砜。
