Hou Luanfeng, Wu Qingping, Gu Qihui, Zhou Qin, Zhang Jumei
Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, No.100 Central Xianlie Road, Guangzhou, 510070, China.
School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China.
Curr Microbiol. 2018 Jul;75(7):918-924. doi: 10.1007/s00284-018-1466-4. Epub 2018 Mar 19.
Aniline has aroused general concern owing to its strong toxicity and widespread distribution in water and soil. In the present study, the bacterial community composition before and after aniline acclimation was investigated. High-throughput Illumina MiSeq sequencing analysis illustrated a large shift in the structure of the bacterial community during the aniline acclimation period. Bacillus, Lactococcus, and Enterococcus were the dominant bacteria in biologically activated carbon before acclimation. However, the proportions of Pseudomonas, Thermomonas, and Acinetobacter increased significantly and several new bacterial taxa appeared after aniline acclimation, indicating that aniline acclimation had a strong impact on the bacterial community structure of biological activated carbon samples. Strain AN-1 accounted for the highest number of colonies on incubation plates and was identified as Acinetobacter sp. according to phylogenetic analysis of the 16S ribosomal ribonucleic acid gene sequence. Strain AN-1 was able to grow on aniline at pH value 4.0-10.0 and showed high aniline-degrading ability at neutral pH.
苯胺因其高毒性以及在水和土壤中的广泛分布而引起了广泛关注。在本研究中，对苯胺驯化前后的细菌群落组成进行了调查。高通量Illumina MiSeq测序分析表明，在苯胺驯化期间细菌群落结构发生了很大变化。驯化前，芽孢杆菌属、乳球菌属和肠球菌属是生物活性炭中的优势细菌。然而，苯胺驯化后，假单胞菌属、嗜热单胞菌属和不动杆菌属的比例显著增加，并且出现了几个新的细菌分类群，这表明苯胺驯化对生物活性炭样品的细菌群落结构有强烈影响。菌株AN-1在培养平板上的菌落数最多，根据16S核糖体核糖核酸基因序列的系统发育分析被鉴定为不动杆菌属。菌株AN-1能够在pH值为4.0 - 10.0的苯胺上生长，并且在中性pH下表现出高苯胺降解能力。
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