Watanabe S, Tani T, Watanabe S, Seno M
Kanagawa Prefectural Public Health Laboratory, Yokohama, Japan.
Lipids. 1990 Oct;25(10):633-8. doi: 10.1007/BF02536014.
The effects of binding of free fatty acids (FFA) to bovine serum albumin (BSA) on steroid hormone binding to BSA were examined. The FFA studied included myristic, palmitic, stearic, oleic and linoleic acids. The binding coefficient K was estimated from the changes in the equilibrium partition coefficient between the aqueous and the hexane phase caused by the addition to BSA to the aqueous phase. A noticeable effect of FFA binding (molar ratio FFA/BSA, 2:1) on the affinities of alpha-estradiol, ethynylestradiol and dehydroisoandrosterone to BSA was not observed: however, the affinities of progesterone, androsterone and testosterone were distinctly enhanced by FFA binding. Furthermore, the elution profiles of gel filtration chromatography clearly showed that progesterone and testosterone are easily liberated from the hormone/BSA complexes and that stronger binding of these hormones to BSA is caused by binding of FFA to BSA. The affinity of ethynylestradiol to BSA is stronger than that of progesterone and testosterone and is not affected by palmitic acid binding to BSA.
研究了游离脂肪酸（FFA）与牛血清白蛋白（BSA）结合对类固醇激素与BSA结合的影响。所研究的FFA包括肉豆蔻酸、棕榈酸、硬脂酸、油酸和亚油酸。结合系数K是根据向水相中添加BSA后水相和己烷相之间平衡分配系数的变化来估算的。未观察到FFA结合（FFA/BSA摩尔比为2:1）对α-雌二醇、乙炔雌二醇和脱氢表雄酮与BSA亲和力的显著影响：然而，FFA结合明显增强了孕酮、雄酮和睾酮的亲和力。此外，凝胶过滤色谱的洗脱曲线清楚地表明，孕酮和睾酮很容易从激素/BSA复合物中释放出来，并且这些激素与BSA的更强结合是由FFA与BSA的结合引起的。乙炔雌二醇与BSA的亲和力强于孕酮和睾酮，且不受棕榈酸与BSA结合的影响。
