Barekati Zeinab, Radpour Ramin, Kohler Corina, Zhong Xiao Yan
Laboratory for Gynecological Oncology, Women's Hospital and Department of Biomedicine, University of Basel, Basel 4031, Switzerland.
Obstet Gynecol Int. 2010;2010. doi: 10.1155/2010/870865. Epub 2010 Jul 27.
DNA methylation is an epigenetic regulation mechanism of genomic function, and aberrant methylation pattern has been found to be a common event in many diseases and human cancers. A large number of cancer studies have been focused on identification of methylation changes as biomarkers (i.e., breast cancer). However, still clinical use of them is very limited because of lack of specificity and sensitivity for diagnostic test. This highlights the critical need for specific primer and probe design to avoid false-positive detection of methylation profiling. The guideline and online web tools that are introduced in this paper might help to perform a successful experiment and to develop specific diagnosis biomarkers by designing right primer pair and probe prior to experimental step.
DNA甲基化是一种基因组功能的表观遗传调控机制，并且已发现异常的甲基化模式在许多疾病和人类癌症中是常见现象。大量癌症研究聚焦于将甲基化变化鉴定为生物标志物（例如乳腺癌）。然而，由于诊断测试缺乏特异性和敏感性，它们在临床中的应用仍然非常有限。这凸显了对特异性引物和探针设计的迫切需求，以避免甲基化谱的假阳性检测。本文介绍的指南和在线网络工具可能有助于在实验步骤之前通过设计正确的引物对和探针来成功进行实验并开发特异性诊断生物标志物。
