通过比率荧光探针选择性和灵敏地检测和定量芳香胺 N-乙酰转移酶 2。
Selective and sensitive detection and quantification of arylamine N-acetyltransferase 2 by a ratiometric fluorescence probe.
机构信息
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, East China University of Science and Technology, Shanghai 200237, China.
出版信息
Chem Commun (Camb). 2010 Oct 14;46(38):7121-3. doi: 10.1039/c0cc01000f. Epub 2010 Aug 26.
PMID:20798904
Abstract
A novel NAT2 specific probe has been developed for sensitive detection of the activity of NAT2. Upon NAT2 catalyzed acetylation of the molecule to N-acetyl-amonafide, the ratiometric response of fluorescence with significant changes in wavelength and intensity is observed. Its significant application potential has been successfully demonstrated in cellular systems.
摘要
已经开发出一种新型的 NAT2 特异性探针，用于灵敏检测 NAT2 的活性。在 NAT2 催化该分子乙酰化形成 N-乙酰-单酰胺时，观察到荧光的比率响应发生显著的波长和强度变化。它在细胞系统中的成功应用潜力已经得到了证明。
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