Department of Biomedical Engineering, Oregon Health & Science University, CH13B, Portland, OR 97239, USA.
Ann Biomed Eng. 2011 Sep;39(9):2329-45. doi: 10.1007/s10439-011-0352-z. Epub 2011 Jul 15.
The effects of flow on endothelial cells (ECs) have been widely examined for the ability of fluid shear stress to alter cell morphology and function; however, the effects of EC morphology without flow have only recently been observed. An increase in lithographic techniques in cell culture spurred a corresponding increase in research aiming to confine cell morphology. These studies lead to a better understanding of how morphology and cytoskeletal configuration affect the structure and function of the cells. This review examines EC micropatterning research by exploring both the many alternative methods used to alter EC morphology and the resulting changes in cellular shape and phenotype. Micropatterning induced changes in EC proliferation, apoptosis, cytoskeletal organization, mechanical properties, and cell functionality. Finally, the ways these cellular manipulation techniques have been applied to biomedical engineering research, including angiogenesis, cell migration, and tissue engineering, are discussed.
细胞培养中微图案化技术的进步
已有大量研究表明，流动对内皮细胞（EC）的影响是通过流体切应力改变细胞形态和功能来实现的；然而，最近才观察到在没有流动的情况下 EC 形态的影响。细胞培养中光刻技术的增加刺激了相应的研究，旨在限制细胞形态。这些研究使人们更好地理解了形态和细胞骨架结构如何影响细胞的结构和功能。本综述通过探索改变 EC 形态的多种替代方法以及由此引起的细胞形状和表型的变化，研究了内皮细胞微图案化研究。微图案化诱导 EC 增殖、凋亡、细胞骨架组织、机械性能和细胞功能的变化。最后，讨论了这些细胞操作技术在生物医学工程研究中的应用，包括血管生成、细胞迁移和组织工程。
