Herath H M T U, Di Silvio L, Evans J R G
Department of Materials, Queen Mary, University of London, London - UK.
J Appl Biomater Biomech. 2005 Sep-Dec;3(3):192-8.
We have determined the biocompatibility of high porosity (92%) sintered hydroxyapatite (HA) foams prepared using a novel ceramic foaming system. The ability of human osteoblast-like cells to grow within the HA foam was investigated in vitro using human osteosarcoma cells seeded directly on the ceramic surfaces to determine the bioactivity. Scanning electron microscopy showed evidence of attachment of numerous cells to the surface. Significant proliferation was observed and the pattern was comparable to that of the tissue culture control, Thermanox TM . There was an increase in cell proliferation and retention of phenotype for the period studied. This hydroxyapaptite foam which has the advantage of being easily fashioned by surgeons, shows potential as a bone substitute scaffold for tissue engineering and future development for clinical application.
我们已经确定了使用新型陶瓷发泡系统制备的高孔隙率（92%）烧结羟基磷灰石（HA）泡沫的生物相容性。使用直接接种在陶瓷表面的人骨肉瘤细胞，在体外研究人成骨样细胞在HA泡沫中生长的能力，以确定其生物活性。扫描电子显微镜显示有大量细胞附着在表面的迹象。观察到显著的增殖，其模式与组织培养对照品Thermanox TM相当。在所研究的时间段内，细胞增殖增加且表型得以保留。这种羟基磷灰石泡沫具有易于外科医生塑形的优点，显示出作为组织工程骨替代支架以及未来临床应用开发的潜力。
