3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal.
Macromol Biosci. 2010 Dec 8;10(12):1495-504. doi: 10.1002/mabi.201000011. Epub 2010 Aug 26.
We report the production of chitosan-based fibers and chitosan fiber-mesh structures by melt processing (solvent-free) to be used as tissue-engineering scaffolds. The melt-based approach used to produce the scaffolds does not change their main characteristics, including the surface roughness and microporosity. The porosity, pore size, interconnectivity and mechanical performance of the scaffolds are all within the range required for various tissue-engineering applications. Biological assessments are performed in direct-contact assays. Cells are able to colonize the scaffold, including the inner porous structure. The cells show high indices of viability in all of the scaffold types.
我们通过熔融加工（无溶剂）制备了壳聚糖纤维和壳聚糖纤维网格结构，将其用作组织工程支架。用于制备支架的熔融方法不会改变它们的主要特性，包括表面粗糙度和微孔性。支架的孔隙率、孔径、连通性和机械性能都在各种组织工程应用所需的范围内。直接接触试验进行生物学评估。细胞能够定殖支架，包括内部多孔结构。细胞在所有支架类型中均显示出高存活率。
