Barykova Iu A, Shmarov M M, Logunov D Iu, Verkhovskaia L V, Aliaev Iu G, Fiev D N, Vinarov A Z, Vinarova N A, Rakovskaia I V, Naroditskiî B S, Gudkov A V, Gintsburg A L
Zh Mikrobiol Epidemiol Immunobiol. 2010 Jul-Aug(4):81-5.
Tests for Mycoplasma hominis, M. genitalium, Ureaplasma urealyticum in males with suspected prostate cancer.
Identification of mycoplasms was performed in prostate tissue samples using universal PCR as well as in serum samples of patients with suspected prostate cancer using ELISA for detection of IgG to M. hominis. Two hundred and fifty samples from each lobe of prostate were obtained from 125 patients with suspected prostate cancer by transrectal polyfocal biopsy. Blood samples were drawn from the same patients for ELISA.
Out of 125 patients with suspected prostate cancer, 20.5% were positive for Mycoplasma by PCR. Between studied species, only M. hominis was found in big proportion of analyzed samples. Out of 118 serum samples, 30.5% were positive for IgG to M. hominis in ELISA.
Fact of presence of Mycoplasma species in tissue of prostate was established in 20.5% pf patients with suspected prostate cancer. Obtained results show that M. hominis is frequently infects prostate tissue and that this infection was more common in patients with high grade prostatic interstitial neoplasia and prostate cancer than in patients with benign changes of prostate tissue or in persons without prostate disease. This allows to suggest that infection with M. hominis could play an important role in development of cancer.
对疑似前列腺癌男性患者进行人型支原体、生殖支原体和解脲脲原体检测。
采用通用聚合酶链反应（PCR）对前列腺组织样本中的支原体进行鉴定，并采用酶联免疫吸附测定（ELISA）检测疑似前列腺癌患者血清样本中人型支原体IgG抗体。通过经直肠多灶活检从125例疑似前列腺癌患者的前列腺各叶获取250份样本。采集同一患者的血液样本进行ELISA检测。
125例疑似前列腺癌患者中，20.5%经PCR检测支原体呈阳性。在所研究的支原体种类中，仅人型支原体在大部分分析样本中被发现。118份血清样本中，30.5%经ELISA检测人型支原体IgG抗体呈阳性。
在20.5%的疑似前列腺癌患者前列腺组织中确定存在支原体。所得结果表明，人型支原体常感染前列腺组织，且这种感染在高级别前列腺间质瘤和前列腺癌患者中比在前列腺组织良性改变患者或无前列腺疾病者中更为常见。这提示人型支原体感染可能在癌症发生中起重要作用。
