Radiation Safety Office, Chung Shan Medical University, Taichung City, Taiwan.
J Agric Food Chem. 2010 Sep 22;58(18):10247-52. doi: 10.1021/jf102379n.
The effects of protocatechuic acid (PCA) on hepatic activity and/or mRNA expression of lipogenic enzymes and sterol regulatory element-binding proteins (SREBPs) in mice fed a trans fatty acid (TFA)-rich diet were examined. PCA at 1, 2, or 4% was provided for 10 weeks. Results showed that TFA diet significantly enhanced hepatic activity and mRNA expression of fatty acid synthase, 3-hydroxy-3-methylglutaryl coenzyme A reductase, stearoyl-CoA desaturase-1, and SREBP-1c (P<0.05); however, the intake of PCA significantly diminished the activity and mRNA expression of these lipogenic factors and decreased hepatic lipid accumulation (P<0.05). TFA diet significantly increased hepatic levels of TFA and pro-inflammatory cytokines (P<0.05). However, PCA intake significantly lowered hepatic content of 18:1 trans and 18:2 trans, as well as reduced the level of test cytokines (P<0.05). These results indicate that PCA is a potent agent for attenuating TFA-induced hepatic steatosis.
本研究旨在探讨原儿茶酸（PCA）对摄入富含反式脂肪酸（TFA）饮食的小鼠肝脏活性和/或脂肪生成酶和固醇调节元件结合蛋白（SREBP）mRNA 表达的影响。实验中，1%、2%或 4%的 PCA 连续喂养 10 周。结果表明，TFA 饮食显著增强了脂肪酸合酶、3-羟-3-甲基戊二酰辅酶 A 还原酶、硬脂酰辅酶 A 去饱和酶-1 和 SREBP-1c 的肝脏活性和 mRNA 表达（P<0.05）；然而，PCA 的摄入显著降低了这些脂肪生成因子的活性和 mRNA 表达，并减少了肝脏脂质堆积（P<0.05）。TFA 饮食显著增加了肝脏中的 TFA 和促炎细胞因子水平（P<0.05）。然而，PCA 的摄入显著降低了 18:1 反式和 18:2 反式的肝脏含量，并降低了细胞因子水平（P<0.05）。这些结果表明，PCA 是一种有效减轻 TFA 诱导的肝脂肪变性的物质。
