Medical University of Vienna, Center of Medical Physics and Biomedical Engineering, Vienna, Austria.
J Biomed Opt. 2010 Jul-Aug;15(4):041513. doi: 10.1117/1.3463008.
An experimental design for noninvasive assessment of neural retinal tissue function with enhanced sensitivity is presented. By matching the response detection to a defined flicker frequency stimulus similar to heterodyne detection, the response signal will be shifted out of the low-frequency noise and the specificity of response detection will be strongly enhanced. Optimal measurement parameters are discussed, such as the function and timing of the response function to a single flash stimulus. The results indicate responses on the order of 200 ms that have been probed with our frequency-encoded approach using 5 Hz flickering. Preliminary results indicate the feasibility of our measurement concept to assess small changes in reflectivity with enhanced sensitivity. A functional tomogram for response localization and quantification is introduced.
提出了一种具有增强灵敏度的非侵入式神经视网膜组织功能评估的实验设计。通过将响应检测与定义的闪烁频率刺激匹配，类似于外差检测，响应信号将移出低频噪声，响应检测的特异性将得到极大增强。讨论了最佳测量参数，例如对单个闪光刺激的响应函数的功能和定时。结果表明，使用我们的 5 Hz 闪烁频率编码方法探测到的响应约为 200 ms。初步结果表明，我们的测量概念具有评估反射率微小变化的可行性，具有增强的灵敏度。引入了用于响应定位和量化的功能断层扫描。
