University of California Davis, Department of Biomedical Engineering, Davis, California 95616, USA.
J Biomed Opt. 2013 Aug;18(8):86010. doi: 10.1117/1.JBO.18.8.086010.
The hemodynamic response to neuronal activation is a well-studied phenomenon in the brain, due to the prevalence of functional magnetic resonance imaging. The retina represents an optically accessible platform for studying lamina-specific neurovascular coupling in the central nervous system; however, due to methodological limitations, this has been challenging to date. We demonstrate techniques for the imaging of visual stimulus-evoked hyperemia in the rat inner retina using Doppler optical coherence tomography (OCT) and OCT angiography. Volumetric imaging with three-dimensional motion correction, en face flow calculation, and normalization of dynamic signal to static signal are techniques that reduce spurious changes caused by motion. We anticipate that OCT imaging of retinal functional hyperemia may yield viable biomarkers in diseases, such as diabetic retinopathy, where the neurovascular unit may be impaired.
由于功能磁共振成像的普及，神经元激活引起的血液动力学反应是大脑中研究得很好的现象。视网膜是研究中枢神经系统中特定层神经血管耦合的一个可及的光学平台；然而，由于方法学上的限制，这在迄今为止一直具有挑战性。我们展示了使用多普勒光相干断层扫描 (OCT) 和 OCT 血管造影术在大鼠内视网膜中成像视觉刺激诱发充血的技术。具有三维运动校正的容积成像、面流计算和将动态信号归一化为静态信号的技术可减少由运动引起的伪变化。我们预计，视网膜功能充血的 OCT 成像可能会为疾病提供可行的生物标志物，例如糖尿病视网膜病变，其中神经血管单元可能受损。
