Randall Division of Cell and Molecular Biophysics, King's College London, New Hunts House, Guys Campus, London, United Kingdom.
Int Rev Cell Mol Biol. 2010;283:57-91. doi: 10.1016/S1937-6448(10)83002-3.
Adhesion to the extracellular matrix (ECM) and to adjacent cells is a fundamental requirement for survival, differentiation, and migration of numerous cell types during both embryonic development and adult homeostasis. Different types of adhesion structures have been classified within different cell types or tissue environments. Much is now known regarding the complexity of protein composition of these critical points of cell contact with the extracellular environment. It has become clear that adhesions are highly ordered, dynamic structures under tight spatial control at the subcellular level to enable localized responses to extracellular cues. However, it is only in the last decade that the relative dynamics of these adhesion proteins have been closely studied. Here, we provide an overview of the recent data arising from such studies of cell-matrix and cell-cell contact and an overview of the imaging strategies that have been developed and implemented to study the intricacies and hierarchy of protein turnover within adhesions.
细胞黏附于细胞外基质（ECM）和相邻细胞是在胚胎发育和成人稳态期间，许多细胞类型生存、分化和迁移的基本要求。在不同的细胞类型或组织环境中，已经对不同类型的黏附结构进行了分类。现在已经清楚的是，这些与细胞外环境接触的关键部位的蛋白质组成非常复杂。很明显，黏附是在亚细胞水平上受到严格空间控制的高度有序、动态的结构，以实现对细胞外信号的局部响应。然而，直到最近十年，这些黏附蛋白的相对动力学才被密切研究。在这里，我们概述了这些关于细胞-基质和细胞-细胞接触的研究中出现的最新数据，以及为研究黏附体中蛋白质周转的复杂性和层次结构而开发和实施的成像策略。
