Institute of Applied Research, Vilnius University, Sauletekio 10, 10223 Vilnius, Lithuania.
J Photochem Photobiol B. 2010 Dec 2;101(3):326-31. doi: 10.1016/j.jphotobiol.2010.08.002. Epub 2010 Aug 10.
This study was focused on the possibility to inactivate thermosensitive Listeria monocytogenes ATC(L3)C 7644 and thermoresistant 56 Ly strain by Na-Chlorophyllin (Na-Chl)-based photosensitization in vitro and on the surface of packaging. Comparative analysis of antimicrobial efficiency of photosensitization with conventional surface cleaning was performed. Data indicate that both Listeria strains, after incubation with Na-Chl and following illumination (λ=400nm, 20mWcm(-2)), were inactivated by 7 log in vitro. This treatment cleaned both Listeria strains from packaging surfaces. Comparative analysis indicates that washing with water diminishes pathogens by less than 1 log, 200ppm Na-hypochlorite by 1.7 log, Na-Chl-based photosensitization by 4.5 log. Listeria biofilms were totally removed from the surface by photosensitization at higher photosensitizer concentrations and longer incubation times. In conclusion, both strains of L. monocytogenes can be effectively inactivated by photosensitization in vitro and on the surface of packaging. Listeria biofilms are susceptible to this treatment as well. Comparison of different surface decontamination treatments reveals that photosensitization is much more effective against both Listeria strains than washing with water or 200ppm Na-hypochlorite. Our data support the idea that Na-Chl-based photosensitization is an effective antimicrobial tool which may serve in the future for the development of human and environmentally friendly surface decontamination techniques.
本研究旨在探讨叶绿酸钠（Na-Chl）基光动力法体外灭活热敏李斯特菌 ATC(L3)C 7644 和耐热 56 Ly 株以及包装表面杀菌的可能性，并对其与传统表面清洁的抗菌效率进行了比较分析。结果表明，李斯特菌经 Na-Chl 孵育并光照（λ=400nm，20mWcm(-2)）后，在体外可分别被灭活 7 个对数。该处理方法可清除包装表面的两种李斯特菌。比较分析表明，水洗涤可减少不到 1 个对数的病原体，200ppm 次氯酸钠减少 1.7 个对数，Na-Chl 基光动力法减少 4.5 个对数。在更高的光敏剂浓度和更长的孵育时间下，李斯特菌生物膜可被光动力法完全从表面去除。结论：两种李斯特菌均可通过体外和包装表面的光动力法有效灭活，李斯特菌生物膜也对此种处理敏感。不同表面消毒处理方法的比较表明，光动力法对两种李斯特菌的杀菌效果均优于水洗涤或 200ppm 次氯酸钠。我们的数据支持 Na-Chl 基光动力法是一种有效的抗菌工具的观点，未来可能用于开发对人类和环境友好的表面消毒技术。
