Institute of Applied Research, Vilnius University, Sauletekio 10, 10223 Vilnius, Lithuania.
J Photochem Photobiol B. 2011 Oct 5;105(1):69-74. doi: 10.1016/j.jphotobiol.2011.06.011. Epub 2011 Jul 23.
The aim of this study was to evaluate efficiency of photosensitization as surface sanitation alternative using model systems when food pathogens, their spores and biofilms were attached to the food-related surface (polyolefine). In addition it was important to compare antibacterial efficiency of Na-Chlorophyllin (Na-Chl)-based photosensitization with conventional sanitizers. Obtained results indicate that Bacilluscereus ATCC 12826 and Listeriamonocytogenes ATCC 7644 as well as their thermoresistant strains B.cereus SV90 and L.monocytogenes 56LY were effectively inactivated (7 log) by Na-Chl-based photosensitization in vitro. Inactivation rate of thermoresistant strains was slower. The number of attached to the surface B.cereus ATCC 12826 and L.monocytogenes ATCC 7644 was reduced from 4-4.5 log to 0 log after photosensitization treatment. To achieve adequate inactivation of thermoresistant strains the higher Na-Chl concentration and longer illumination times had to be used. Comparison of different surface decontamination treatments reveal that photosensitization is much more effective against all surface-attached B.cereus and L.monocytogenes strains than washing with water or 200 ppm Na-hypochlorite. It is important to note, that surface-attached B.cereus spores and L.monocytogenes biofilms can be eliminated from it by photosensitization as well. Our data support the idea that Na-Chlorophyllin-based photosensitization has high antibacterial potential which may serve in the future for the development of human and environment friendly, non-thermal surface decontamination technique.
本研究的目的是评估模型系统中光致敏作用作为表面卫生替代方法的效率，当食源性病原体、其孢子和生物膜附着在与食品相关的表面（聚烯烃）上时。此外，比较基于 Na-叶绿素（Na-Chl）的光致敏作用与传统消毒剂的抗菌效率也很重要。结果表明，Bacillus cereus ATCC 12826 和 Listeria monocytogenes ATCC 7644 及其耐热菌株 B.cereus SV90 和 L.monocytogenes 56LY 均可通过体外 Na-Chl 基光致敏作用有效灭活（7 log）。耐热菌株的灭活率较慢。附着在表面的 B.cereus ATCC 12826 和 L.monocytogenes ATCC 7644 的数量在光致敏处理后从 4-4.5 log 减少到 0 log。为了实现对耐热菌株的充分灭活，必须使用更高的 Na-Chl 浓度和更长的光照时间。不同表面去污处理的比较表明，光致敏作用对所有附着在表面的 B.cereus 和 L.monocytogenes 菌株都比水或 200 ppm Na-次氯酸盐洗涤更有效。值得注意的是，附着在表面的 B.cereus 孢子和 L.monocytogenes 生物膜也可以通过光致敏作用从表面消除。我们的数据支持这样一种观点，即基于 Na-叶绿素的光致敏作用具有很高的抗菌潜力，未来可能用于开发对人类和环境友好的非热表面去污技术。
