Department of Applied Life science, Kon Kuk University, Seoul, South Korea.
Hum Exp Toxicol. 2011 Aug;30(8):904-9. doi: 10.1177/0960327110382565. Epub 2010 Aug 27.
This study was conducted to evaluate the in vitro scavenging activity and inhibitory effect of low-density lipoprotein oxidation of pro-oxidant reactive species in response to treatment with organic solvent fractions from mung bean (seeds of Vigna radiata) extracts using various screening methods including biological and non-biological oxidants. The antioxidant activity of different organic solvent fractions from mung bean extracts were studied in vitro methods by evaluating the total phenol components, measuring the antioxidant activity by trolox equivalent antioxidant capacity, measuring the scavenging effects on reactive oxygen species and on reactive nitrogen species as well as measuring the inhibitory effect on Cu(2+)-induced human low-density lipoprotein oxidation. The mung bean extracts were found to have a potent scavenging activity against all of the reactive species tested as well as an inhibitory effect on low-density lipoprotein oxidation, the studied mung bean was revealed to be very effective against the evaluated pro-oxidant species, including reactive oxygen species and on reactive nitrogen species.
本研究旨在评估绿豆（Vigna radiata 种子）提取物的有机溶剂级分处理后，对生物和非生物氧化剂的反应中，活性氧物种的体外清除活性和低密度脂蛋白氧化的抑制作用。采用多种筛选方法，包括生物和非生物氧化剂，用体外方法研究了不同有机溶剂级分的绿豆提取物的抗氧化活性。通过评估总酚成分、用 Trolox 当量抗氧化能力测量抗氧化活性、测量对活性氧和活性氮的清除作用以及测量对 Cu(2+)-诱导的人低密度脂蛋白氧化的抑制作用，来评估不同有机溶剂级分的绿豆提取物的抗氧化活性。研究发现，绿豆提取物对所有测试的活性物质都具有很强的清除活性，并且对低密度脂蛋白氧化具有抑制作用。研究表明，绿豆对所评估的促氧化剂物质（包括活性氧和活性氮）非常有效。
