College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Food Chem. 2012 Sep 15;134(2):1030-7. doi: 10.1016/j.foodchem.2012.03.009. Epub 2012 Mar 12.
Forty samples were extracted from defatted kernels and hulls of two varieties of hempseed (Bama and Yunma No. 1) using 10 different polar solvent systems. The radical scavenging capacity of the extracts was evaluated using 2,2-diphenyl-1-pikrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays and the total phenolic content was determined by Folin-Ciocalteu's phenol reagent. The correlation analysis indicated that the antioxidants in hempseed belonged to phenolic and DPPH() assay was suitable for evaluating the radical scavenging activity. Two compounds, with predominant antiradical activity, were isolated in 60% ethanol extract of hempseed hull using macroporous resin absorption, LH-20 gel chromatography, and high performance liquid chromatography methods, which were identified as N-trans-caffeoyltyramine and cannabisin B by high-resolution mass spectra, nuclear magnetic resonance spectra, and ultraviolet data. The two compounds exhibited significant high DPPH() scavenging activity and protective effect against in vitro oxidation of human low-density lipoprotein compared with extracts from flaxseed, grape seed, and soybean. This suggests that hempseed hull extract is a potential source of natural antioxidants, which could be added to dietary supplements to help prevent oxidative stress.
从两个品种的大麻种子（巴马和云麻 1 号）的脱脂仁壳中提取了 40 个样本，使用 10 种不同的极性溶剂系统。使用 2,2-二苯基-1-苦基肼（DPPH）和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）测定法评估提取物的自由基清除能力，并通过福林-考尔莱特酚试剂测定总酚含量。相关性分析表明，大麻种子中的抗氧化剂属于酚类物质，DPPH（）测定法适用于评估自由基清除活性。两种具有主要抗自由基活性的化合物，使用大孔树脂吸附、LH-20 凝胶色谱和高效液相色谱方法，从大麻种子壳的 60%乙醇提取物中分离出来，通过高分辨率质谱、核磁共振谱和紫外数据鉴定为 N-反式-咖啡酰酪胺和大麻素 B。与亚麻籽、葡萄籽和大豆提取物相比，这两种化合物表现出显著的高 DPPH（）清除活性和对体外人低密度脂蛋白氧化的保护作用。这表明大麻种子壳提取物是天然抗氧化剂的潜在来源，可添加到膳食补充剂中，以帮助预防氧化应激。
