Vestibular Neurogenetics Laboratory, Boys Town National Research Hospital, Omaha, Nebraska 68131, USA.
Dev Dyn. 2010 Oct;239(10):2659-73. doi: 10.1002/dvdy.22405.
Otoconia, developed during late gestation and perinatal stages, couple mechanic force to the sensory hair cells in the vestibule for motion detection and bodily balance. In the present work, we have investigated whether compensatory deposition of another protein(s) may have taken place to partially alleviate the detrimental effects of Oc90 deletion by analyzing a comprehensive list of plausible candidates, and have found a drastic increase in the deposition of Sparc-like 1 (aka Sc1 or hevin) in Oc90 null versus wt otoconia. We show that such up-regulation is specific to Sc1, and that stable transfection of Oc90 and Sc1 full-length expression constructs in NIH/3T3 cells indeed promotes matrix calcification. Analysis and modeling of Oc90 and Sc1 protein structures show common features that may be critical requirements for the otoconial matrix backbone protein. Such information will serve as the foundation for future regenerative purposes.
耳石在胚胎后期和围产期发育，将机械力传递给前庭感觉毛细胞，以进行运动检测和身体平衡。在本工作中，我们通过分析一整套可能的候选蛋白，研究了是否发生了另一种（或多种）蛋白的代偿性沉积，以部分减轻 Oc90 缺失的有害影响，发现 Oc90 缺失的耳石中 Sparc 样 1（又名 Sc1 或 hevin）的沉积明显增加。我们表明这种上调是 Sc1 特有的，并且在 NIH/3T3 细胞中稳定转染 Oc90 和 Sc1 全长表达构建体确实促进了基质钙化。对 Oc90 和 Sc1 蛋白结构的分析和建模显示出共同的特征，这些特征可能是耳石基质骨干蛋白的关键要求。这些信息将为未来的再生目的提供基础。
