Hodde K C, Steeber D A, Albrecht R M
Laboratory for Experimental Surgery, Academic Medical Center, Amsterdam, The Netherlands.
Scanning Microsc. 1990 Sep;4(3):693-704.
This paper briefly discusses the concept of corrosion cast preparation (primarily of blood vessels), the use of the scanning electron microscope (SEM) to study these casts and the observations which can be made, together with the merits and the limitations in various applications. A number of reviews and surveys are quoted in which the different injection media, injection methods, animal preparations and corrosion procedures are described. A new procedure of cleaning the corrosion casts with sodium hydroxide and Triton X-100 is described. The observations which can be made are listed and illustrated both on the cellular level as well as in organ systems as a whole. The discussion centers around some common misconceptions, the feasibility in various applications and the limitations of the method. The conclusion is that the method has proven to be useful especially in conjunction with other methods. Moreover, while the concept of the method may be very straight-forward the approach and the interpretation often need careful consideration and might not be as straight-forward as one tends to expect from the simple sounding principle.
本文简要讨论了腐蚀铸型制备（主要是血管的）概念、使用扫描电子显微镜（SEM）研究这些铸型以及所能进行的观察，同时还探讨了其在各种应用中的优点和局限性。文中引用了一些综述和调查，其中描述了不同的注射介质、注射方法、动物标本制备和腐蚀程序。介绍了一种用氢氧化钠和曲拉通X-100清洗腐蚀铸型的新方法。列出并说明了在细胞水平以及整个器官系统中所能进行的观察。讨论围绕一些常见的误解、该方法在各种应用中的可行性以及局限性展开。结论是，该方法已被证明是有用的，特别是与其他方法结合使用时。此外，虽然该方法的概念可能非常简单直接，但方法和解释往往需要仔细考虑，可能不像人们从简单的原理中预期的那样直接。
