牛磺酸和粒细胞集落刺激因子对谷氨酸诱导的原代皮质神经元兴奋毒性的保护作用。
Protection of taurine and granulocyte colony-stimulating factor against excitotoxicity induced by glutamate in primary cortical neurons.
机构信息
Department of Chemistry and Biochemistry, Florida Atlantic University, Boca Raton, FL 33431, USA.
出版信息
J Biomed Sci. 2010 Aug 24;17 Suppl 1(Suppl 1):S18. doi: 10.1186/1423-0127-17-S1-S18.
BACKGROUND
Both taurine, an inhibitory neurotransmitter and granulocyte colony-stimulating factor (G-CSF), a growth factor, possess neuroprotective and neurotrophic properties in vitro. However, the mechanisms of their underlying neuroprotective effects are not fully understood.
METHODS
In the present study, we investigated the potential protective benefits of taurine, G-CSF and the combination of taurine and G-CSF against excitotoxicity induced by glutamate in primary cortical neuronal cultures.
RESULTS
25 mM taurine, 25 ng/ml G-CSF and the combination of 25 mM taurine and 25 ng/ml G-CSF showed a protective effect reaching 75%, 75% and 88%, respectively. Furthermore, taurine exerted its protective effect through down-regulation of expression of GRP 78, CHOP, Bim and caspase 12.
CONCLUSION
The results showed that all of these treatments, taurine, G-CSF and the combination of taurine and G-CSF, protected primary cortical neurons against excitotoxicity induced by glutamate. ER stress is suppressed by taurine after glutamate toxicity.
背景
牛磺酸是一种抑制性神经递质，粒细胞集落刺激因子（G-CSF）是一种生长因子，它们在体外均具有神经保护和神经营养作用。然而，其潜在神经保护作用的机制尚不完全清楚。
方法
本研究旨在探讨牛磺酸、G-CSF 以及牛磺酸和 G-CSF 联合应用对原代皮质神经元培养物中谷氨酸诱导的兴奋性毒性的潜在保护作用。
结果
25mM 牛磺酸、25ng/ml G-CSF 和 25mM 牛磺酸和 25ng/ml G-CSF 的联合应用分别达到 75%、75%和 88%的保护作用。此外，牛磺酸通过下调 GRP78、CHOP、Bim 和 caspase12 的表达发挥其保护作用。
结论
这些治疗方法，包括牛磺酸、G-CSF 和牛磺酸与 G-CSF 的联合应用，均能保护原代皮质神经元免受谷氨酸诱导的兴奋性毒性。牛磺酸在谷氨酸毒性后抑制内质网应激。
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