Department of Ophthalmology, Juntendo University School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
Exp Eye Res. 2010 Nov;91(5):721-6. doi: 10.1016/j.exer.2010.08.020. Epub 2010 Sep 6.
This study evaluated the distribution of goblet cells and the expression of MUC5AC mRNA in the canine nictitating membrane. The distribution of goblet cells in the nictitating membrane and temporal bulbar conjunctiva of beagle dogs was examined by histochemical analysis of impression cytology specimens and frozen sections. MUC5AC mRNA was detected by the reverse transcription polymerase chain reaction (RT-PCR). The distribution of MUC5AC mRNA was also examined by in situ hybridization using digoxigenin-labeled antisense and sense RNA probes. Histochemical analysis showed that the canine nictitating membrane epithelium contained many more periodic acid-Schiff positive goblet cells, particularly on the palpebral side, compared with the temporal bulbar conjunctiva. RT-PCR revealed that MUC5AC was expressed in both the nictitating membrane and in conjunctival tissue. When the distribution of MUC5AC mRNA was assessed by in situ hybridization, its expression was high on the palpebral side of the nictitating membrane and low in the temporal bulbar conjunctiva. MUC5AC mRNA expression corresponded with the distribution of goblet cells by histochemical examination. In conclusion, there were numerous goblet cells in the canine nictitating membrane epithelium, particularly on the palpebral side, and MUC5AC mRNA was expressed in the nictitating membrane epithelium at locations corresponding to the goblet cells.
本研究评估了杯状细胞在犬瞬膜中的分布以及 MUC5AC mRNA 的表达。通过对印迹细胞学标本和冷冻切片的组织化学分析，检查了瞬膜和比布鲁斯球结膜中杯状细胞的分布。通过逆转录聚合酶链反应 (RT-PCR) 检测 MUC5AC mRNA。通过使用地高辛标记的反义和正义 RNA 探针进行原位杂交，也检查了 MUC5AC mRNA 的分布。组织化学分析表明，与比布鲁斯球结膜相比，犬瞬膜上皮含有更多的过碘酸-希夫阳性杯状细胞，特别是在睑侧。RT-PCR 显示 MUC5AC 在瞬膜和结膜组织中均有表达。通过原位杂交评估 MUC5AC mRNA 的分布时，其在瞬膜的睑侧表达较高，而在比布鲁斯球结膜的颞侧表达较低。MUC5AC mRNA 的表达与组织化学检查中杯状细胞的分布相对应。总之，犬瞬膜上皮中有大量杯状细胞，特别是在睑侧，MUC5AC mRNA 在与杯状细胞相对应的瞬膜上皮中表达。
