Department of Rehabilitation for the Movement Functions, Research Institute, National Rehabilitation Center for Persons with Disabilities, Tokorozawa, Saitama, Japan.
Spinal Cord. 2011 Mar;49(3):333-6. doi: 10.1038/sc.2010.116. Epub 2010 Aug 31.
An in vivo study in a rat model of acute spinal cord contusion.
To assess the efficacy of novel therapies for acute spinal cord injury (SCI), methods to evaluate accurately the effects of these therapies should be developed. Although neurological examination is commonly used for this purpose, unstable clinical conditions and the spontaneous recovery of neurological function in the acute and subacute phases after injury make this measurement unreliable. Recent studies have reported that the phosphorylated form of the high-molecular-weight neurofilament subunit NF-H (pNF-H), a new biomarker for axonal degeneration, can be measured in serum samples in experimental SCI animals. Therefore, we aimed to investigate the use of plasma pNF-H as an indicator of the efficacy of minocycline, a neuroprotective drug, for treating SCI.
This study was carried out at Saitama, Japan.
Spinal cord injured rats received either minocycline or saline intraperitoneally. The plasma pNF-H levels and functional hind limb score were determined after the injury.
Minocycline treatment reduced plasma pNF-H levels at 3 and 4 days post-injury (dpi). Rats with lower plasma pNF-H levels at 3 dpi had higher hind limb motor score at 28 dpi.
pNF-H levels may serve as a biomarker for evaluating the efficacy of therapies for SCI.
急性脊髓挫伤大鼠模型的体内研究。
评估急性脊髓损伤 (SCI) 新疗法的疗效，应开发出准确评估这些疗法效果的方法。尽管神经学检查常用于此目的，但不稳定的临床状况和损伤后急性和亚急性期神经功能的自发恢复使得这种测量不可靠。最近的研究报告称，血清样本中磷酸化的高分子量神经丝亚单位 NF-H（pNF-H），一种轴突退化的新生物标志物，可以在实验性 SCI 动物中测量。因此，我们旨在研究血浆 pNF-H 作为一种指标，用于评估米诺环素（一种神经保护药物）治疗 SCI 的疗效。
本研究在日本埼玉进行。
脊髓损伤大鼠通过腹腔内给予米诺环素或生理盐水。损伤后测定血浆 pNF-H 水平和后肢功能评分。
米诺环素治疗可降低损伤后 3 天和 4 天的血浆 pNF-H 水平。在 3 天有较低血浆 pNF-H 水平的大鼠，在 28 天具有更高的后肢运动评分。
pNF-H 水平可能作为评估 SCI 治疗效果的生物标志物。
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