Lindén C, Alm A
Department of Ophthalmology, University Hospital, Umeå, Sweden.
Acta Ophthalmol (Copenh). 1990 Dec;68(6):633-8. doi: 10.1111/j.1755-3768.1990.tb01686.x.
The effect of reduced tear drainage on the intraocular penetration of topically applied fluorescein was studied with fluorophotometry in healthy eyes. Corneal and aqueous fluorescein concentrations could not be followed for the first hour after application of fluorescein because tear film fluorescense influenced fluorophotometric determinations of fluorescein concentrations in cornea and aqueous for at least 30 min. Insertion of punctal plugs in the upper and lower punctum of one eye caused a significant (P less than 0.025) increase in aqueous fluorescein concentrations 1 to 8 h after application of 20 microliters of a 2% solution of sodium fluorescein in the lower conjunctival sac. The achieved concentrations were almost 4 times as large as in the fellow eye. There was some individual variation, but a marked increase was observed in 9 of 11 subjects. Attempts to reduce tear drainage by compressing the tear sac and/or closing the eye lids for 1 min after application of the eye drop had no significant effect on corneal or aqueous concentrations of fluorescein 1 to 8 h later.
在健康眼睛中，使用荧光光度法研究了泪液引流减少对局部应用荧光素眼内渗透的影响。应用荧光素后的第一个小时内，无法跟踪角膜和房水荧光素浓度，因为泪膜荧光至少在30分钟内影响了荧光光度法对角膜和房水中荧光素浓度的测定。在一只眼睛的上下泪点插入泪点塞，在结膜下囊滴入20微升2%荧光素钠溶液后1至8小时，房水荧光素浓度显著（P小于0.025）升高。所达到的浓度几乎是另一只眼睛的4倍。存在一些个体差异，但11名受试者中有9名观察到明显升高。滴眼后通过压迫泪囊和/或闭眼1分钟来减少泪液引流的尝试，对1至8小时后角膜或房水中荧光素的浓度没有显著影响。
