靶向可还原氧化的蛋白酪氨酸磷酸酶超家族。
Targeting the reversibly oxidized protein tyrosine phosphatase superfamily.
机构信息
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
出版信息
Sci Signal. 2010 Aug 31;3(137):pl2. doi: 10.1126/scisignal.3137pl2.
Abstract
Controlled production of reactive oxygen species leads to reversible oxidation of protein tyrosine phosphatases (PTPs) and has emerged as an important tier of regulation over phosphorylation-dependent signal transduction. We present a modified cysteinyl-labeling assay that detects reversible oxidation of members of each of the different PTP subclasses. Here, we describe the methods for enriching reversibly oxidized PTPs from complex protein extracts, illustrating the procedure in IMR90 fibroblasts.
摘要
可控的活性氧物质的产生导致蛋白酪氨酸磷酸酶（PTPs）的可逆氧化，并且已经成为依赖于磷酸化的信号转导的调控的一个重要层次。我们提出了一种改良的半胱氨酸标记测定法，用于检测不同 PTP 亚类的成员的可逆氧化。在这里，我们描述了从复杂蛋白质提取物中富集可逆氧化的 PTP 的方法，并用 IMR90 成纤维细胞说明了该程序。
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