Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, 85 Wujin Road, Shanghai 200080, People's Republic of China.
Br J Cancer. 2010 Sep 28;103(7):961-9. doi: 10.1038/sj.bjc.6605870. Epub 2010 Aug 31.
Our recent study observed that the expression of ubiquitin D (UBD), a member of ubiquitin-like modifier family, was upregulated in colon cancer parenchymal cells. The present study further investigated the clinical signicance of UBD in colon cancer.
Using quantitative PCR, tissue microarray (TMA), western blot analysis and immunohistochemical stain, we evaluated UBD mRNA and protein levels in tumour tissues from patients with colon cancer at different stages and in paired adjacent normal epithelium.
Immunohistochemical detection of UBD on a TMA containing 203 paired specimens showed that increased cytoplasmic UBD was signicantly associated with depth of cancer invasion, lymph node metastasis, distant metastasis, tumour histologic grade, advanced clinical stage and Ki-67 proliferative index. Patients with UBD-positive tumours had a significantly higher disease recurrence rate and poorer survival than patients with UBD-negative tumours after the radical surgery. Stratification analysis according to tumour stage revealed UBD as an independent predictor for tumour recurrence in patients with stage II and III tumours.
UBD may contribute to the progression of colon carcinogenesis and function as a novel prognostic indicator of forecasting recurrence of stage II and III patients after curative operations.
我们最近的研究观察到，泛素 D（UBD）的表达在结肠癌实质细胞中上调，泛素 D 是泛素样修饰家族的成员。本研究进一步探讨了 UBD 在结肠癌中的临床意义。
采用定量 PCR、组织微阵列（TMA）、western blot 分析和免疫组织化学染色，我们评估了不同阶段结肠癌患者肿瘤组织中和配对的相邻正常上皮组织中 UBD mRNA 和蛋白水平。
在包含 203 对标本的 TMA 上进行 UBD 的免疫组织化学检测显示，细胞质 UBD 的增加与癌症浸润深度、淋巴结转移、远处转移、肿瘤组织学分级、晚期临床分期和 Ki-67 增殖指数显著相关。在根治性手术后，UBD 阳性肿瘤患者的疾病复发率明显高于 UBD 阴性肿瘤患者，且生存较差。根据肿瘤分期进行分层分析显示，UBD 是 II 期和 III 期肿瘤患者肿瘤复发的独立预测因子。
UBD 可能有助于结肠癌发生进展，并可作为预测 II 期和 III 期患者根治术后复发的新型预后指标。
