Laboratory of Nematology, Wageningen University, Wageningen, The Netherlands.
PLoS One. 2010 Aug 16;5(8):e12145. doi: 10.1371/journal.pone.0012145.
Organophosphorus pesticides (OPs) were originally designed to affect the nervous system by inhibiting the enzyme acetylcholinesterase, an important regulator of the neurotransmitter acetylcholine. Over the past years evidence is mounting that these compounds affect many other processes. Little is known, however, about gene expression responses against OPs in the nematode Caenorhabditis elegans. This is surprising because C. elegans is extensively used as a model species in toxicity studies. To address this question we performed a microarray study in C. elegans which was exposed for 72 hrs to two widely used Ops, chlorpyrifos and diazinon, and a low dose mixture of these two compounds. Our analysis revealed transcriptional responses related to detoxification, stress, innate immunity, and transport and metabolism of lipids in all treatments. We found that for both compounds as well as in the mixture, these processes were regulated by different gene transcripts. Our results illustrate intense, and unexpected crosstalk between gene pathways in response to chlorpyrifos and diazinon in C. elegans.
有机磷农药（OPs）最初是通过抑制乙酰胆碱酯酶这种神经递质乙酰胆碱的重要调节剂来影响神经系统的。近年来，越来越多的证据表明，这些化合物还会影响许多其他过程。然而，人们对秀丽隐杆线虫（C. elegans）中针对 OPs 的基因表达反应知之甚少。这令人惊讶，因为 C. elegans 被广泛用作毒性研究的模式生物。为了解决这个问题，我们对秀丽隐杆线虫进行了微阵列研究，该线虫在 72 小时内暴露于两种广泛使用的 OPs，即毒死蜱和二嗪农，以及这两种化合物的低剂量混合物中。我们的分析揭示了所有处理中与解毒、应激、先天免疫以及脂质的运输和代谢相关的转录反应。我们发现，对于这两种化合物以及混合物，这些过程是由不同的基因转录物调节的。我们的结果说明了在 C. elegans 中，对毒死蜱和二嗪农的反应中存在强烈的、意想不到的基因途径交叉对话。
