Laboratory of Nematology, Wageningen University, Wageningen, The Netherlands.
PLoS One. 2011;6(9):e24676. doi: 10.1371/journal.pone.0024676. Epub 2011 Sep 9.
Although organophosphorus pesticides (OP) share a common mode of action, there is increased awareness that they elicit a diverse range of gene expression responses. As yet however, there is no clear understanding of these responses and how they interact with ambient environmental conditions. In the present study, we investigated genome-wide gene expression profiles in the nematode Caenorhabditis elegans exposed to two OP, chlorpyrifos and diazinon, in single and combined treatments at different temperatures. Our results show that chlorpyrifos and diazinon induced expression of different genes and that temperature affected the response of detoxification genes to the pesticides. The analysis of transcriptional responses to a combination of chlorpyrifos and diazinon shows interactions between toxicants that affect gene expression. Furthermore, our combined analysis of the transcriptional responses to OP at different temperatures suggests that the combination of OP and high temperatures affect detoxification genes and modified the toxic levels of the pesticides.
尽管有机磷农药 (OP) 具有共同的作用模式，但人们越来越意识到它们会引起多种基因表达反应。然而，目前对于这些反应以及它们如何与环境条件相互作用还没有明确的认识。在本研究中，我们研究了在不同温度下，用两种 OP（毒死蜱和二嗪农）进行单一和联合处理时，线虫秀丽隐杆线虫的全基因组基因表达谱。我们的结果表明，毒死蜱和二嗪农诱导了不同基因的表达，而温度影响了解毒基因对杀虫剂的反应。对毒死蜱和二嗪农混合物的转录反应分析表明，有毒物质之间存在相互作用，影响基因表达。此外，我们对不同温度下 OP 的转录反应的综合分析表明，OP 和高温的组合会影响解毒基因，并改变杀虫剂的毒性水平。
