Szczypka M, Gajewski J, Laskowska-Klita T, Zbieg-Sendecka E
National Research Institute of Mother and Child, Department of Biochemistry, Warsaw, Poland.
Biomed Biochim Acta. 1990;49(10):1015-9.
Many reports have pointed out that oxidative damage and disturbances in antioxidant defense systems of the lenses may play an important role in the development of cataract. In the present study the activities of glutathione peroxidase, glutathione reductase, glutathione-S-transferase, glucose-6-phosphate dehydrogenase, catalase and the level of glutathione and lipid peroxides were measured in red blood cells of galactosaemic children with cataract and without cataract. Furthermore the serum antioxidant activity and the level of uric acid. ceruloplasmin and transferrin in serum were estimated. It was found that in red blood cells of galactosaemic children with cataract the activity of glutathione reductase was slightly lower than in a control age-matched group of children and in galactosaemic children without cataract. The increase of serum antioxidant activity in both groups of galactosaemic children was also observed. Probably it could be due to the increase of the level of ceruloplasmin. Except glutathione reductase activity no other differences were found in the investigated components of the antioxidant defense systems of red blood cells and serum between galactosaemic children with cataract and those without cataract. Therefore it seems that red blood cells and serum metabolism are no good reflections of disturbances in antioxidant defense mechanisms which may be involved in the cataract development in galactosaemic children.
许多报告指出，晶状体的氧化损伤及抗氧化防御系统紊乱可能在白内障的发生发展中起重要作用。在本研究中，测定了患有白内障和未患白内障的半乳糖血症儿童红细胞中谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽-S-转移酶、葡萄糖-6-磷酸脱氢酶、过氧化氢酶的活性以及谷胱甘肽和脂质过氧化物的水平。此外，还评估了血清抗氧化活性以及血清中尿酸、铜蓝蛋白和转铁蛋白的水平。结果发现，患有白内障的半乳糖血症儿童红细胞中谷胱甘肽还原酶的活性略低于年龄匹配的对照组儿童以及未患白内障的半乳糖血症儿童。在两组半乳糖血症儿童中均观察到血清抗氧化活性增加。这可能是由于铜蓝蛋白水平升高所致。除谷胱甘肽还原酶活性外，在患有白内障和未患白内障的半乳糖血症儿童红细胞和血清抗氧化防御系统的其他研究成分中未发现差异。因此，红细胞和血清代谢似乎不能很好地反映可能参与半乳糖血症儿童白内障发生的抗氧化防御机制的紊乱情况。
