Department and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Int J Legal Med. 2011 Mar;125(2):219-26. doi: 10.1007/s00414-010-0500-2. Epub 2010 Aug 31.
Interest in the development of polymorphic short tandem repeat (STR) markers unlinked to the CODIS loci is growing among forensic practitioners. We developed a multiplex system in which14 autosomal STR (D3S1744, D4S2366, D8S1110, D12S1090, D13S765, D14S608, Penta E, D17S1294, D18S536, D18S1270, D20S470, D21S1437, Penta D, and D22S683) could be amplified in one single polymerase chain reaction. DNA samples from 572 unrelated Taiwanese Han subjects were analyzed using this 14 STR multiplex system. Thirty parent-child pairs of parentage testing cases with a combined paternity index (CPI) below 1,000 and 32 parent-child pairs with single-step mutations found in AmpFℓSTR Identifiler loci were also recruited for validation of the newly developed system. DNA sequencing was performed for novel STRs and novel alleles found in these subjects. The distributions of allelic frequencies for these autosomal STRs and sequence data, allele nomenclature for the STRs, and forensic parameters are presented. The discrimination power in our multiplex loci ranged from 0.6858 (D18S536) to 0.9168 (Penta E), with a combined discrimination power of 0.999999999. It provides additional power to distinguish the possible single-step mutations in parent-child pairs and improves the ability to prove parentage by increasing the CPI. The combined power of exclusion of these 14 loci in Taiwanese Han in this study was 0.9999995913. In conclusion, this 14-autosomal STRs multiplex system provides highly informative STR data and appears useful in forensic casework and parentage testing.
法医学家越来越关注与 CODIS 基因座无关的多态性短串联重复序列（STR）标记的开发。我们开发了一种多重扩增系统，可以在单个聚合酶链反应中扩增 14 个常染色体 STR（D3S1744、D4S2366、D8S1110、D12S1090、D13S765、D14S608、Penta E、D17S1294、D18S536、D18S1270、D20S470、D21S1437、Penta D 和 D22S683）。使用该 14 个 STR 多重扩增系统对 572 名无关的台湾汉族个体的 DNA 样本进行了分析。还招募了 30 对亲权鉴定案例的亲子对（CPI 低于 1000）和 32 对在 AmpFℓSTR Identifiler 基因座中发现的单步突变亲子对，以验证新开发的系统。对这些个体中发现的新 STR 和新等位基因进行 DNA 测序。本文介绍了这些常染色体 STR 的等位基因频率分布、STR 序列数据、STR 等位基因命名法以及法医参数。本研究中多重扩增基因座的鉴别能力范围为 0.6858（D18S536）至 0.9168（Penta E），综合鉴别能力为 0.999999999。它提供了额外的能力来区分亲子对中可能的单步突变，并通过增加 CPI 提高了亲子关系证明的能力。在这项研究中，台湾汉族个体的 14 个排除位点的综合排除能力为 0.9999995913。总之，该 14 个常染色体 STR 多重扩增系统提供了高度信息丰富的 STR 数据，在法医学和亲子关系鉴定中似乎很有用。
